Sel MCF-7 merupakan salah satu model sel kanker payudara yang banyak digunakan dalam penelitian. Sel tersebut diambil dari jaringan payudara seorang wanita Kaukasian berumur 69 tahun golongan darah O, dengan Rh positif, berupa sel adherent (melekat) yang dapat ditumbuhkan dalam media penumbuh DMEM atau RPMI yang mengandung foetal bovine serum (FBS) 10% dan antibiotik Penicilin-Streptomycin 1% (Anonim, 2007). Sel MCF-7 memiliki karakteristik antara lain resisten agen kemoterapi (Mechetner et al., 1998; Aouali et al., 2003), mengekspresikan reseptor estrogen (ER +), overekspresi Bcl-2 (Butt et al., 2000; Amundson et al., 2000) dan tidak mengekspresikan caspase-3 (Onuki et al., 2003; Prunet et al., 2005). Sel MCF-7 tergolong cell line adherent (ATCC, 2008) yang mengekspresikan reseptor estrogen alfa (ER-α), resisten terhadap doxorubicin (Zampieri dkk., 2002), dan tidak mengekspresikan caspase-3 (Onuki dkk., 2003; Prunet dkk., 2005).
Gambar 1. Morfologi sel MCF-7 pada perlakuan EP dan FKP. Uji dilakukan dengan menginkubasi 5×103 sel MCF-7 dengan EP (25-100 µg/mL) dan FKP (10-500 µg/mL) selama 48 jam. A adalah kontrol sel, B adalah sel dengan perlakuan EP 75 µg/mL, C adalah sel dengan perlakuan FKP 70 µg/mL
Daftar Pustaka :
Amundson, S.A., Myers, T.G., Scudiero, D., Kitada, S., Reed, J.C., and Fornace, A.J., 2000, An Informatics Approach Identifying Markers of Chemosensitivity in Human Cancer Cell Lines, Cancer Res, 60:6101-6110.
Anonim, 2007, ATCC Cell Biology, available from http://www.atcc.org/common /catalog/numSearch/numResults.cfm?atcc Num=HTB-22, cited in 25 June 2007.
Aouali, N., Morjani, H., Trussardi, A., Soma, E., Giroux, B., and Manfait, M., 2003, Enhanced Cytotoxicity and Nuclear Accumulation of Doxorubicin-loaded Nanospheres in Human Breast Cancer MCF-7 Cells Expressing MRP1, International Journal of Oncology, 23:1195-1201.
ATCC, 2008, Cell Biology, ATCC® Number: HTB-22TM, Designations: MCF-7, http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=HTB-22&Template=cellBiology, 19 Juli 2008.
Butt, A.J., Firth, S.M., King, M.A., and Baxter, R.C., 2000, Insulin-Like Growth Factor-Binding Protein-3 Modulates Expression of Bax and Bcl-2 and Potentiates P53-Independent Radiation-Induced Apoptosis In Human Breast Cancer Cells, J. Biol Chem, 275(50):39174-39181.
Menchetner, E., Kyshtoobayeva, A., Zonis, S., Kim, H., Stroup, R., Garcia, R., Parker, R.J., and Fruehauf, J.P., 1998, Levels of Multidrug Resistance (MDR1) P-Glycoprotein Expression by Human Breast Cancer Correlate with in Vitro Resistance to Taxol and Doxorubicin, Clinical Cancer Research, 4:389-398.
Onuki, R., Kawasaki, H., Baba, T., dan Taira, K., 2003, Analysis of A Mitochondrial Apoptotic Pathway Using Bid-Targeted Ribozymes in Human MCF7 Cells in the Absence of A Caspase-3-Dependent Pathway, Antisense and Nucleic Acid Drug Development, 13 (2): 75-82.
Prunet, C., Lemaire-Ewing, S., Ménétrier, F., Néel, D., dan Lizard, G., 2005, Activation of Caspase-3-Dependent and -Independent Pathways During 7-Ketocholesterol- and 7β-Hydroxycholesterol-Induced Cell Death: A Morphological and Biochemical Study, Journal of Biochemical and Molecular Toxicology, 19 (5): 311-326.
Zampieri, L., Bianchi, P., Ruff, P., dan Arbuthnot, P., 2002, Differential Modulation by Estradiol of P-glycoprotein Drug Resistance Protein Expression in Cultured MCF7 and T47D Breast Cancer Cells, Anticancer Res., 22 (4): 2253-9.
Kontributor :
Sandro Rossano Yunas, Sri Handayani dan Adam Hermawan
