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MCF-7 Cell

MCF-7 cells is one of the breast cancer cell models that are widely used in research. The cells taken from a woman’s breast tissue 69-year-old Caucasian blood group O, Rh positive, form in adherent cells (attached) which can be grown in DMEM or RPMI medium grower containing fetal bovine serum (FBS) and 10% Penicillin-Streptomycin antibiotics 1% (Anonim, 2007). MCF-7 cells have characteristics include resistance chemotherapeutic agents (Mechetner et al., 1998; Aouali et al., 2003), express estrogen receptors (ER +), overexpression of Bcl-2 (Butt et al., 2000; Amundson et al., 2000) and do not express caspase-3 (Onuki et al., 2003; Prunet et al., 2005). MCF-7 cells classified as adherent cell line (ATCC, 2008) that express the estrogen receptor alpha (ER-α), resistant to doxorubicin (Zampieri dkk., 2002), and do not express caspase-3 (Onuki dkk., 2003; Prunet dkk., 2005).

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Gambar 1. MCF-7 cell morphology in EP treatment and FKP. Tests were carried out by incubating 5 × 103 cells MCF-7 with EP (25-100 µg / mL) and FKP (10-500 µg / mL) for 48 hours. A is the control cells, B cells were treated with EP 75 µg/mL, C is the treatment of cells with FKP 70 µg/mL

References :

Amundson, S.A., Myers, T.G., Scudiero, D., Kitada, S., Reed, J.C., and Fornace, A.J., 2000, An Informatics Approach Identifying Markers of Chemosensitivity in Human Cancer Cell Lines, Cancer Res, 60:6101-6110.

Anonim, 2007, ATCC Cell Biology, available from http://www.atcc.org/common /catalog/numSearch/numResults.cfm?atcc Num=HTB-22, cited in 25 June 2007.

Aouali, N., Morjani, H., Trussardi, A., Soma, E., Giroux, B., and Manfait, M., 2003, Enhanced Cytotoxicity and Nuclear Accumulation of Doxorubicin-loaded Nanospheres in Human Breast Cancer MCF-7 Cells Expressing MRP1, International Journal of Oncology, 23:1195-1201.

ATCC, 2008, Cell Biology, ATCC® Number: HTB-22TM, Designations: MCF-7, http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=HTB-22&Template=cellBiology, 19 Juli 2008.

Butt, A.J., Firth, S.M., King, M.A., and Baxter, R.C., 2000, Insulin-Like Growth Factor-Binding Protein-3 Modulates Expression of Bax and Bcl-2 and Potentiates P53-Independent Radiation-Induced Apoptosis In Human Breast Cancer Cells, J. Biol Chem, 275(50):39174-39181.

Menchetner, E., Kyshtoobayeva, A., Zonis, S., Kim, H., Stroup, R., Garcia, R., Parker, R.J., and Fruehauf, J.P., 1998, Levels of Multidrug Resistance (MDR1) P-Glycoprotein Expression by Human Breast Cancer Correlate with in Vitro Resistance to Taxol and Doxorubicin, Clinical Cancer Research, 4:389-398.

Onuki, R., Kawasaki, H., Baba, T., dan Taira, K., 2003, Analysis of A Mitochondrial Apoptotic Pathway Using Bid-Targeted Ribozymes in Human MCF7 Cells in the Absence of A Caspase-3-Dependent Pathway, Antisense and Nucleic Acid Drug Development, 13 (2): 75-82.


Prunet, C., Lemaire-Ewing, S., Ménétrier, F., Néel, D., dan Lizard, G., 2005, Activation of Caspase-3-Dependent and -Independent Pathways During 7-Ketocholesterol- and 7β-Hydroxycholesterol-Induced Cell Death: A Morphological and Biochemical Study, Journal of Biochemical and Molecular Toxicology, 19 (5): 311-326.

Zampieri, L., Bianchi, P., Ruff, P., dan Arbuthnot, P., 2002, Differential Modulation by Estradiol of P-glycoprotein Drug Resistance Protein Expression in Cultured MCF7 and T47D Breast Cancer Cells, Anticancer Res., 22 (4): 2253-9.

Contributors :

Sandro Rossano Yunas, Sri Handayani dan Adam Hermawan