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WiDr Cell

WiDr cells (Figure 1) is a human colon cancer cells were isolated from the colon of a 78-year-old woman. WiDr cells are derived the other colon cancer cells namely HT-29 cells (Chen et al., 1987). Carcinoembryonic antigen WiDr cells produce and require a time span of about 15 hours to complete one cell cycle. One of the characteristics of the WiDr cells is the expression sikolooksigenase-2 (COX-2) high stimulate WiDr cell proliferation (Palozza et al., 2005) A at position 273 that changes an arginine residueinto a histidine à. In WiDr cells occurring mutation p53 G (Noguchi et al., 1979). However, p21 in normal cells WiDr that still allows for the discontinuation the cell cycle (Liu et al., 2006). Apoptosis in WiDr cells can occur through a pathway independent of p53, including through the activation of p73 (Levrero et al., 2000).

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Figure 1. WiDr cells a day after thawing (A) and after reaching confluent (B and C).

WiDr cell is one that has a low sensitivity to treatment with 5-fluorouracil (5-FU), antimetabolite class of chemotherapeutic agents. WiDr transfection with normal p53 also did not cause an increase in sensitivity to 5-FU (Giovannetti et al., 2007). WiDr cell resistance to 5-FUone of which is mediated by theincreased expression ofthymidylate synthetase enzyme which is the main target of the inhibition of 5-FU (Sigmond et al., 2003). P-glycoprotein (PGP) in WiDr cells do not express highso that there may be other mechanisms that mediate resistance to 5-FU WiDr (Jansen, 1997). Overall, WiDr cell is a cell that is suitable for use as a model in screening new compounds as co-chemotherapy agents with 5-FU.

References :

Chen, T.R., Drabkowski, D., Hay, R.J., Macy, M. and Peterson, W. Jr., 1987, WiDr is a Derivative of Another Colon Adenocarcinoma Cell Line, HT-29, Cancer Genet Cytogenet., 27(1):125-34.

Giovannetti, E., Backus, H.H.J., Wouters, D., Ferreira, C.G., van Houten, V.M.M. and Brakenhoff, R.H., 2007, Changes in the Status of p53 Affect Drug Sensitivity to Thymidylate Synthase (TS) Inhibitors by Altering TS Levels, British J. Can., 96:769-775.

Jansen, W.J.M., Zwart, B., Hulscher, S.T.M., Giaccone, Pinedo, H.M. and Boven, E., 1997, CPT-11 in Human Colon-Cancer Cell Lines and Xenografts: Characterization of Cellular Sensitivity Determinants, Int. J. Cancer, 70:335-340.

Levrero, M., Laurenzi, V. De, Constanzo, A., Sabatini, S., Gong, J., Wang, J.Y.J. and Melino, G., 2000, The p53/p63/p73 Family of Transcription Factors: Overlapping and Distinct Functions, Journal of Cell Science, 113:1661-1670.

Liu, H.C., Chen, G.G., Vlantis, A.C., Leung, B.C.S., Tong, M.C.E. and van Hasselt, C.A., 2006, 5-Fluorouracil Mediates Apoptosis and G1/S Arrest in Laryngeal Squamous Cell Carcinoma via a p53-Independent Pathway, The Cancer Journal, 12(6):482-493.

Noguchi, P., Wallace, R., Johnson, J., Early, E.M., O’Brien, S. and Ferrone, S., 1979, Characterization of the WiDr: a Human Colon Carcinoma Cell Line, In Vitro, 15(6):401-408.

Palozza, P., Serini, S., Maggiano, N., Giuseppe, T., Navarra, P. and Ranelletti, F.O., 2005, -Carotene Downregulates the Steady-State and Heregulin-a-Induced COX-2 Pathways in Colon Cancer Cells, J.Nutr., 135:129-136.

Sigmond, J., Backus, H.H., Wouters, D., Temmink, O.H., Jansen, G. and Peters, G.J., 2003, Induction of Resistance to the Multitargeted Antifolate Pemetrexed (ALIMTA) in WiDr Human Colon Cancer Cells is Associated with Thymidilate Synthase Overexpression, Biochem. Pharmacol.,

Contributors :

Endah Puji Septisetyani dan Adam Hermawan